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Can Assisted Hatching Improve the Chances of IVF Success?
Couples who have considered IVF would have been introduced to the term assisted hatching. Many clinics offer assisted hatching as a part of their IVF treatment at a certain cost or include it as a free option if they believe that it can be beneficial. Although the procedure hasn’t shown to increase the rates of live birth, it is recommended by many clinics. If your doctor has suggested it to you, here is some information about assisted hatching that you should know.
What is Assisted Hatching?
The embryo before fertilisation starts as an egg or an oocyte. All eggs are surrounded by a protein shell that is known as the zona pellucida. Before the egg becomes an embryo, a fusion of the zona pellucida with a sperm cell at the beginning of the fertilisation process needs to occur. After the first sperm cell gets through the zona pellucida to fertilise the egg, the shell becomes hard and impenetrable to prevent the rest of the sperm cells from penetrating to the fertilised zygote. The shell hardening also ensures the embryo doesn’t implant in the fallopian tube prematurely. It also keeps the dividing blastocyst cells safe as they make their way to the uterus.
The zygote continues to develop into a mass of cells called a blastocyst as it travels through the fallopian tube to the uterus. Throughout this process, the zona pellucida continues to thin out and expand, and eventually deteriorates. Around the fourth day of travel, the deteriorated shells crack open similar to an egg so the blastocyst or embryo can emerge. The thin zona pellucida is left behind completing the hatching process of the embryo. A few days after hatching, the blastocyst proceeds to implant itself to the uterine wall or endometrium. Without the hatching process, blastocysts cannot implant themselves into the uterine wall. This may result in failure of the pregnancy.
Fertilisation in IVF treatments occurs inside a lab and women who have undergone IVF treatment know that having a fertilised embryo doesn’t mean pregnancy is guaranteed. The transferred embryo has to implant or “stick” successfully into the endometrium for them to get pregnant. It is seen that about 85% of transferred embryos do not implant. One of the reasons for this is that the embryos do not hatch properly, hence fail to implant. It could happen due to various other factors such as the shell being unusually hard, or factors like the cultures for the embryos or the cryopreservation chemicals could have interfered with the hatching.
The role of assisted hatching during IVF treatments is to aid in the process of embryo hatching, so the chances of implantation are increased.
Who Can Benefit from Assisted Hatching?
Assisted hatching was developed when it was observed that embryos with a thinner zona pellucida had higher rates of implantation in IVF treatments. Keeping that in mind, the procedure is useful for:
Women over 35 undergoing IVF treatment.
Couples who have had repeated failures at pregnancy after a few IVF cycles.
Embryos where a distinctively thick zona is observed by the embryologist.
What Are the Methods of Assisted Hatching?
The main idea behind assisted hatching is to make a tear or breach in the zona pellucida. Several methods are employed to do this that are mentioned below:
1. Mechanical Hatching
This is the most basic of all procedures where the embryo is kept steady using a pipette. A micro-needle is used to puncture the zona pellucida. The puncture is done in a way that the needle goes through the shell tangentially beneath the shell to make an opening. The region between one end of the puncture and the other is carefully stroked until a small tear occurs. Although the procedure is simple, controlling the tear in this method is difficult.
2. Physical Expansion of the Zona Pellucida
In this procedure, the shell of the embryo is not torn or ruptured mechanically. It is manipulated to undergo a process of expansion which would occur in all shells naturally. The expansion is done through the introduction of a hydrostatic pressure under the shell using a fine needle which causes the zona pellucida to expand.
3. Chemical Hatching
Chemical hatching is a procedure slightly different from the mechanical methods of rupturing the zona pellucida. In this procedure, small amounts of a chemical called Tyrode’s acid is introduced over the shell until it is breached. The embryo is then quickly cleaned to remove the acid to avoid any unnecessary exposure.
4. Drilling
With the drilling technique, microscopic vibrations are focused on the shell to make a conical opening. Vibrations are generated using a method derived from Piezo technology.
4. Laser-assisted Hatching
Laser-assisted hatching involves the usage of a laser device to make an extremely precise opening in the zona pellucida. Since lasers are used, it is possible to have extremely precise control over the size of the breach in the shell. It is the most effective and safest method as compared to all the above procedures. However, many clinics are not equipped with this technology.
Assisted Hatching Procedure
Here is what you need to know more about the assisted hatching procedure:
Assisted hatching came about around 1990 as a way to improve the chances of implantation during IVF treatments.
The procedure is performed three days after fertilisation when the embryo has had a few days to develop.
Assisted hatching involves opening up the zona pellucida which covers the embryo by thinning or rupturing it.
Various techniques are used in the hatching, and some procedures involve dissolving the shell using an acid mixture. A fine needle or laser technique is used to break open the shell.
The procedure involves the use of advanced instruments such as microscopes, microscopic tools, and robotic assistance for the micromanipulation of the minuscule embryo.
The hatched embryo is transferred into the woman’s uterus a day after the procedure.
Antibiotics and steroids may be administered to reduce complications.
Possible Risks
Since the procedure involves manipulation of the embryo and interference with its natural developmental process, there are some assisted hatching ivf risks involved with the procedure. The major risk factor associated with the procedure is that the embryo could sustain lethal damage. It could occur at any time before or after the embryo transfer, the result of either case being a failure of pregnancy.
Twinning is another risk associated with assisted hatching, specifically the monozygotic type. Monozygotic twins, most commonly referred to as identical twins are born out of single egg and sperm. IVF treatments typically have an increased chance of twinning and added to that, hatching might elevate that risk further. Although most multiple pregnancies are risky, pregnancies with monozygotic twins have a greater risk to both the mother and the baby.
Birth defects are another risk factor tied to assisted hatching and many wonder if it increases the chances of the defects. Studies have shown that assisted reproductive technologies carry with them an increased risk for nonchromosomal birth defects. However, the risk of congenital disabilities did not significantly increase in embryos which had procedures such as assisted hatching performed on them, in comparison to other IVF treatments that did not use assisted hatching.
What Is the Cost of Assisted Hatching in India?
Being one of the most commonly used procedures, the cost of laser-assisted hatching in India can range from INR 15,000 to INR 30,000 depending on the fertility clinic.
Although assisted hatching is a procedure that was developed as a way to improve the chances of implantation, nothing can be said about its efficacy. Several factors go into the success of the procedure such as the method used by the clinic, experience level of the embryologist, and the conditions of the embryo.
Also Read:
Foods to Eat and Avoid for IVF Success
Egg Retrieval in IVF
What are the Causes of IVF Failure Read more
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"लखनवी नवाब" के यह 6 स्टाइलिश अंदाज़
बॉलीवुड में “खान” का जादू हमेशा से रहा है, पिछले कई सालों से प्रख्यात 3 खान - सलमान खान , शाहरुख खान और आमिर खान को तो आप बहुत अच्छे से जानते ही हैं। परन्तु इस बार हम उनकी बात नहीं कर रहे हैं, इस बार हम एक विशेष खान की बात कर रहे हैं, जो अभी 2 साल के भी नहीं हुए हैं। जी हाँ! हम सैफीना के बेटे तैमूर अली खान की बात कर रहे हैं, कुछ तो बात है इस नन्हे नवाब में कि एक झलक में ही दुनिया दीवानी हो जाती है। जन्म से ही इनकी ख्याति मीडिया में छाई हुई है, सबके चहेते बनते जा रहे हैं नन्हे मिया।
तैमूर की लुभा देनेवाली उपस्तिथि तथा उनका आत्मविश्वास जो दिखकर आता है, विशेषकर कैमरे के सामने उसका श्रेय उनकी प्यारी सी सूरत को तो जाता ही है परन्तु जिस स्टाइल के कपड़े वे पहनते हैं उसको भी पूरा श्रेय जाता है। तैमूर की आकर्षक आकृति उनके स्टाइलिश दिखनेवाले कपड़ों से बनती है, एक बार आप भी अपनाएं अपने बच्चों के लिए। आप भी अपने नन्हें सरकार को स्टाइल आइकॉन बना सकतीं हैं, तो फिर देर किस बात की? तैमूर को देख कर सिर्फ “काश!!” कहना छोड़िए, और जानिए कहाँ से पा सकती हैं आप अपने लाडले के लिए वैसे ही स्टाइलिश कपड़े। तैमूर के कुछ लुक्स और कपड़ों का स्टाइल, जानें यहां;
1. कैज़ुअल टी शर्ट और 3/4 शॉर्ट्स - आरामदायक पोशाक सैर पर जाने के लिए
आपके बच्चे के लिए बेहतरीन टी शर्ट और शॉर्ट्स, यह पोशाक आपके बच्चे की शारीरिक सुविधा को ध्यान में रख कर ही बनाई गई है । इन कपड़ों में आपका लाडला कैसे भी उठे, बैठे, चले, दौड़े या कूदे भी उसे कोई असुविधा नहीं होगी और वह आरामदायक पल व्यतीत कर सकेगा। कैज़ुअल दिखने वाली यह पोशाक आपके बेटे को एक आकर्षक लुक देगी, और अगर आप अपने बच्चे को यह पोशाक कैनवास जूतों के साथ पहनाएंगी तो वह और भी कूल लगेगा। इस पोशाक में 2 टी शर्ट्स हैं, पहली टी शर्ट फुल है और ग्रे रंग में है तथा दूसरी टी शर्ट हाफ है और काले रंग में है, आप इसे किसी भी बेहतरीन स्टाइल में अपने बच्चे को पहना सकतीं हैं।
[caption id="attachment_202627" align="aligncenter" width="533"] Source: https://www.instagram.com/p/Bqb45XtAc5C/[/caption]
2. आपके शहज़ादे के लिए स्टाइलिश डंगरी
डेनिम की यह डंगरी हमेशा से ही पसंदीदा पोशाक रही है, चाहे वो 90 के दशक में हो या आज के ज़माने में। आपके बच्चे पर यह पोशाक उतनी ही जचेगी जितनी तैमूर पटौदी पर जंच रही है, एक बार पहना कर ज़रूर देखें। रंगो के तालमेल से इस पोशाक का पूरा सेट आपके बच्चे पर खूब जचेगा। इस पोशाक को आप पूरे सेट के साथ भी खरीद सकतीं हैं या फिर आप चाहें तो सिर्फ टी शर्ट या डंगरी, अपनी पसंद के रंगो के हिसाब से भी खरीद सकतीं हैं।
[caption id="attachment_202628" align="aligncenter" width="533"] Source: https://www.instagram.com/p/Bb4ASG1DJMR/[/caption]
3. ट्रेंडी शर्ट स्टाइल - बच्चों पर भी भाये बड़ों का लुक
बच्चों को अगर बड़ों का लुक दिया जाए तो सच में बहुत प्यारे लगते हैं तथा यह सुंदरता और भी बढ़ जाती है जब पहनी हुई पोशाक आरामदेह हो। सफ़ेद रंग की लाइनिंग शर्ट और नीली जीन्स तैमूर पर जितनी लुभा रही है, उतनी ही यह आरामदायक पोशाक भी लग रही है। जिस तरह से नन्हे नवाब ने इसे पहना हुआ है, वे इस पोशाक में कितने सुकून और संयमित लग रहे हैं। सफ़ेद लाइनिंग शर्ट के साथ नीली जीन्स तथा स्नीकर जूते, आपके बच्चे के व्यक्तित्व में निखार लाएगा और वह हर तरह से सम्पूर्ण महसूस करेगा। किसी भी फंक्शन या पार्टी में आप यह पोशाक अपने लाडले को पहना सकतीं हैं, उसकी मासूमियत और स्टाइल से लोगों का ध्यान भटकाने के लिए बस इतना काफी है।
[caption id="attachment_202622" align="aligncenter" width="533"] Source: https://www.instagram.com/p/BdscD4QFHS1/[/caption]
4. नए साल और क्रिसमस में अपनाएं यह यूनीक देसी अंदाज़
जितनी सुंदरता भारतीय संस्कृति में है, उतनी ही सुंदरता भारतीय पोशाक में भी होती है। यह आपने लखनवी नवाब के नए अंदाज़ में देख ही लिया होगा, आरामदेह कुर्ते पायजामे और कोटी में जंच रहे हैं। सिर्फ तैमूर ही नहीं आप अपने बच्चे को भी यह पोशाक पहनाएंगी तो उसे भी काला टीका तो लगाना ही होगा ना? ऐसा नहीं है कि सिर्फ पश्चिमी पोशाक ही बच्चों पर अच्छी लगती है, एक बार भारतीय पोशाक को भी परखें, आपका लाल सभी लोगों का दिल जीत लेगा। बंद गले की जैकेट के साथ कुर्ते का सेट आप कई नए रंगों और नई बनावट में भी अपना कर सकतीं हैं।
[caption id="attachment_202623" align="aligncenter" width="533"] Source: https://www.instagram.com/p/Bc7HsUvlmiV/[/caption]
5. ठण्ड के मौसम में स्टाइल भी और सुरक्षा भी
सर्दियों में अक्सर आप बच्चे को ठंड से बचाने के लिए उसके स्टाइल या फैशन में ध्यान नहीं दे पातीं हैं। आप उसे मोटे स्वेटर और जैकेट पहनाकर बस सुरक्षित रखने की कोशिश करतीं हैं, आप भी सहीं हैं। अगर आपको अपने बच्चे की सर्दी से सुरक्षा के साथ साथ स्टाइल का भी ध्यान रखना है तो हुडी के साथ जैकेट बेहतरीन मेल रहेगा। जैकेट की गर्माहट आपके बच्चे को ठण्ड से बचाएगी तथा हुडी लाएगा आपके बच्चे के लुक में शानदार स्टाइल। यह एक उत्तम कॉम्बिनेशन है आपके बच्चे के फैशन के लिए।
[caption id="attachment_202626" align="aligncenter" width="533"] Source: https://www.instagram.com/p/zBcwbgcyHe8y/[/caption]
6. नन्हे सरकार का उत्तम स्पोर्टी लुक
"एथ्लेज़र" आज का उभरता हुआ नया फैशन है युवावस्था के लिए, पर कौन कहता है कि बच्चे इस फैशन को नहीं अपना सकते? 2 वर्षीय लखनवी नवाब का यह स्पोर्टी अंदाज़ बिल्कुल अलग है। जर्सी टीज़, ट्रैक पैंट व स्पोर्ट जूतों में एक अलग लुक में नज़र आ रहे हैं तैमूर, कल्पना कीजिए आपके बच्चे का व्यक्तित्व भी इस लुक में कितना दृढ़ तथा स्पोर्टी दिखेगा।”नज़र न लगे किसी की” ऐसा ही बोलेंगी आप अपने बच्चे को देख कर, इस पोशाक में आपके बच्चे को भी बाहर खेलने और घूमने में आनंद आएगा, तो फिर देर किस बात की बना दीजिए अपने बेटे को स्पोर्ट आइकॉन।
[caption id="attachment_202624" align="aligncenter" width="533"] Source: https://www.instagram.com/p/BngPo5KhijJ/[/caption]
करीना ने तैमूर को बना दिया स्टाइल आइकॉन, अब आपकी बारी। आप भी बनाएं अपने नन्हे मुन्ने को स्टाइल आइकॉन, अपने अंदाज़ और अपनी पसंद में। Read more
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Laced Chocolate Ganache Cake
One piece of this laced chocolate ganache cake can bring an instant smile on your kid’s face. Chocolate cakes are always a hit among kids. But, the one we are sharing here is not your regular chocolate cake – it is something special. Covered in decadent chocolate ganache and wrapped with a chocolate lace, it is sure to win your kid’s heart instantly
Serves
Preparation Time
Cooking Time
8 People
20 - 25 Minutes
25 - 30 Minutes
Ingredients
2/3 cup maida
½ cup condensed milk
¼ cup butter
3 tbsp cocoa powder
3/4 tsp baking powder
½ tsp baking soda
100 ml soda water
½ tsp vanilla essence
For the Ganache
60 gram dark chocolate, chopped
60 ml cream
For the chocolate lace
50 gram chocolate
Chocolate shavings for garnishing
Method
Step 1
Preheat the oven at 180 C. Grease a 6 inch round tin and dust it with flour.
Step 2
In a mixing bowl, shift all the dry ingredients and stir well. In another medium mixing bowl, add the softened butter and condensed milk and whisk for two minutes. Add the vanilla essence and whisk it well again.
Step 3
Now add the liquid mixture to the dry mixture, slowly and keep whisking to form a lump-free batter. Add the soda water and mix for another 2 minutes.
Step 4
Pour the batter in pre-greased tin and bake it at 180 degrees celsius in the preheated oven for 25-30 minutes. When done a skewer pricked in the middle of the cake will come out clean.
Step 5
Cool it completely on a wire rack. While it is cooling prepare the ganache.
Step 6
Boil cream in a saucepan and add the chopped chocolate into it. Stir and take it off heat. Keep stirring till you get a smooth chocolatey ganache. Let it cool it for 7-10 minutes and pour the ganache over the cooled cake. Spread the ganache evenly.
Step 7
Make a cone with parchment paper. Also cut a strip of parchment paper that fits into the circumference of the cake.
Step 8
Chop the chocolate and melt it in a microwave. Let it cool for a minute or so and fill the paper cone with melted chocolate. Straighten both the ends of the parchment paper and using the parchment paper cone, pipe a lace border chocolate.
Step 9
When the piped border is semi set – it will take 3-4 minutes; carefully wrap it around the cake. Transfer the cake in the refrigerator for 5 minutes. After 5 minutes, take it out and peel off the parchment paper to get a beautifully set lace border.
Step 10
Slice and serve!
Nutritional Information
Calories
233 K cal
Proteins
4.1 g
Fats
12.2 g
Carbohydrates
28.1 g
Cholesterol
26 mg
Sodium
158 mg
Potassium
236 mg
Read more
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Is it too late and risky being pregnant after 35?
Is it too late and risky being pregnant after 35?..I personally know so many who is having this doubt.some of them even put a full stop on their wish for a second baby, only coz they are above 30.. especially 35 !!
If same question asked to me, I would say NO...it's my personal opinion..I too became a mother to my second lovely baby, in my 30 s, especially in my 39 th!! Yes!..and I didn't had to face anything more than from what a 20 s pregnant lady has to face..like vomiting, slight bp variation , chord issue etc..and same issues and much more were faced by other pregnant ladies who were with me at that time, who were on their 20s...
In today's world, most of the ladies gets married around 26 , settles in life, then only thinks about their babies..and first baby comes near to their 30s. And it's unavoidable that their second comes after 35 only.
But there are things which we should keep in mind while planning.
First and important thing is whether we want this baby? Whether you really enjoy being a mother of a baby at this time.
Next is we should make sure we are healthy enough to give birth to a healthy baby.As you are growing older ,your health also must be in risk. So you must make sure about your health.If we are not healthy enough, it might cause problems to our new born. So if we are, our baby will be too!
In respect to our physical health, mental health matters equal importance.If you find yourself older to be a mother to a new born baby, you are spoiling the lively childhood memories of your baby.!.So you must be mentally young enough to be a mother of a kid, spend time, play with them..etc..don't make them feel they are born to a grandma !!..
And above all, avoid what society says !!..It's your decision , your life , your family , and your baby !!!
And am really happy that I became a reason that someone who put the full stop on their dreams for a second baby, erased that full stop and took initiative and is now enjoying their second pregnancy in late life.!!... Read more
Nikitha has added a new answer
Expecting Mom due in 5 months
6 hours ago
Q. hello good afternoon ma'am, Mera 1st trimester khtm ho gya h ,4th month chal raha hai actually mjhe kch dino se bhuk ni lgti h ,khana dkh k Mann ni krta h khane ka toh mai aap kch solution btaye
Nikitha
Mom of a 9 yr 5 m old boy
6 hours ago
A. hello it s normal during the pregnancy and it is absolutely nothing to worry and try to have small meal frequently and avoid taking a large meal at a time which can help you and any concern you can talk with you doctor she can guide you accordingly
Abhaya Rajput has added a new answer
Mom of a 8 m old child
6 hours ago
Q. mera baby 10 month ka ho gya h abi tk khada ni ho para kya jru
Abhaya Rajput
Mom of a 14 yr 4 m old boy
6 hours ago
A. Hello there ma’am please do not worry about it too much. Babies start to stand between the age of 9 months to thirteen months. Please wait for sometime before consulting a doctor. Please give semi solid food items like banana and mashed potatoes and mashed apples to the baby. Also give suji kheer and daal paani to the baby. Continue giving formula milk to the baby. You can also give mashed idli to the baby. Masaage baby legs with BabyHug coconut oil.
Thanks and take care
shilpi Bhandari has added a new answer
Trying To Conceive
4 hours ago
Q. assembly job vacancy in firstcry store
shilpi Bhandari
Mom of 2 children
4 hours ago
A. hello dear this u need to ask them personally on call ot u can mail them as it is a parenting app so u can't right guidance on this here
Prachi Agrawal has added a new answer
Guardian of 0 children
4 hours ago
Q. Hello doctor,
my son is 2.5 months old and completely on breastfeeding. He hasn't pooped since three days. please advise
Prachi Agrawal
Mom of a 1 yr 6 m old girl
2 hours ago
A. After two months of age, babies may have constipation problem which is not concerning until they are showing any abnormal signs.
It may last upto 10 days too. Keep a check on baby's activity and feeding, till the time that is all fine, no worries.
Dr. Minal Acharya has added a new answer
Guardian of 0 children
2 hours ago
Q. i eat chips daily. will it harm my baby?? actually i dont like to eat chapatti
Dr. Minal Acharya
Nutritionist
1 hour ago
A. dear Mum, if you eat chips daily then it will not harm your baby but it will be harmful for your health. so, you should not have it daily. you can have it occasionally.
Dr Ghouse has added a new answer
Expecting Mom due in 2 months
26 mins ago
Q. I am 31 week pregnant, my BP is shooted to 160, as per growth scan baby weight is less 1.4gm, under <.99 percentile. can you suggest me what to do ,or how much weight is expected
Dr Ghouse
Paediatrician
21 mins ago
A. please take medicines to control your blood pressure as advised even regarding induction of labour also. if there is no relief it is better you see your doctor for proper examination particularly physical examination if there is need for doing investigations to find out the problem and treatment ok
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Guardian of a 1 m old girl
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